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分辨率/分离系数（Rs：Resolution）
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- 表彰两个峰的分离度，出峰差和峰宽进行比较，Rs=1表明2个峰刚好分离，可节约时间，Rs＜1分离度不行
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- ![[层析分辨率.jpg]]
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- 分辨率优化的两个方向
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- 选择性（分辨率分子）
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- 填料配基的选择
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- 目标分子和吸附配基的结合条件优化（结合液性质）
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- 分离条件优化（洗脱液性质和洗脱梯度）
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- 图片
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- 有效性（分辨率的分母）
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- 填料基架颗粒直径的选择（颗粒直径越小峰越尖）
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- 良好的柱填装技术（层析柱的柱效）
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- 目标分子的体积大小，溶液的粘度
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- 运行的流速
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- 图片
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