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# 构建细胞/工程菌时需要考虑纯化
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# 表达位置
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分胞内、胞外
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hGH为分泌间质表达
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表达位置决定了破碎的方法
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# 冻融目的
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使细胞松散
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# 超滤
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先超滤后盐析，因为体积大
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经验值：目的分子（大分子）÷膜孔（5KD，hGh）=1.5倍
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# 盐析
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破坏蛋白亲水性水化膜，暴露疏水核
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影响因素
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蛋白浓度，体系中盐的浓度
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过饱和盐：全部蛋白
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不饱和盐：有目的筛选
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