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0.04M NaCl：结合能力减弱，杂质流穿
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0.1M NaCl：目的蛋白
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M NaCl：洗脱结合杂蛋白
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> DEAE1
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> 2021年6月24日 21:48
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- DEAE1
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- 目的
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□
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> 捕获目的蛋白
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- 层析原理
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- 离子交换（弱阴离子交换），低盐上样，高盐洗脱
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- 层析柱型号
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柱内直径（cm）
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柱高（cm）
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柱床体积（L）
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柱床高度（cm）
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上样量mg/ml
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蛋白处理范围（g）
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INDEX 200/500
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20
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50
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8.5±0.5
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25±5
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25\~50
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200\~450
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> ○
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- 填料信息
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- 名称：DEAE Sephrose F.F
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- 更换时间
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- 柱效要求
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2014年
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- 每米理论塔板数：≥2000
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□ 对称性：0.8\~1.2
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- 缓冲液
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- 平衡缓冲液
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- 0.3mol/L Tris-HCl-30m mol/L EDTA
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- 0.04mol/L NaCl-10m mol/L Tris-HCl-1m mol/L EDTA
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- 样品调节缓冲液
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- 0.04mol/L NaCl -10m mol/L Tris-HCl-1m mol/L EDTA
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- 冲洗缓冲液
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- 0.04mol/L NaCl-10m mol/L Tris-HCl-1m mol/L EDTA
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- 洗脱缓冲液
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- 0.1mol/L NaCl-10m mol/L Tris-HCl-1m mol/L EDTA
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- Q&A
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- 装柱比较困难
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> 分区 层析 的第 1 页
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