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33 lines
838 B
33 lines
838 B
2 years ago
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蛋白质分离纯化的一般原则
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- 蛋白质的分离步骤
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- 生物组织的机械破碎
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- 研磨法
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- 超声波法
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- 冻融法
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- 酶解法
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- 根据蛋白质的特性，选择不同的溶剂进行抽提
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- 水溶性蛋白：中性缓冲液抽提
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- 酸性蛋白：稀碱性溶液抽提
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- 脂溶性蛋白：表面活性剂抽提
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- 粗提
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- 离心去除固体杂质后，可通过沉淀法、超滤法、萃取法等处理，得到蛋白质制品
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- 精制
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- 可用层析法、电泳法等进行精制
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- 成品加工
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- 测定蛋白质的性质并干燥成成品
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