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test/紫外分光光度计.md

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  • 吸收光谱法基本定律 物质对单色光吸收的强弱与吸收物质的浓度（C）和液层厚度（l）间的关系的定律，是光吸收的基本定律，是紫外-可见光度法定量的基础。
  • Lamber-Beer定律 当一束平行的单色光通过溶液时，溶液的吸光度（A）与溶液的浓度（C）和光程（b）成正比。 一般情况下光程（b）=液层厚度（l） A=ECl A：吸光度 E：吸光系数，吸光物质在单位浓度及单位厚度时的吸光度 c：浓度： l：厚度，单位cm