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分辨率/分离系数RsResolution

-   表彰两个峰的分离度出峰差和峰宽进行比较Rs=1表明2个峰刚好分离可节约时间Rs1分离度不行
  • !层析分辨率.jpg

  • 分辨率优化的两个方向

    • 选择性(分辨率分子)

      • 填料配基的选择

      • 目标分子和吸附配基的结合条件优化(结合液性质)

      • 分离条件优化(洗脱液性质和洗脱梯度)

      • 图片

  • 有效性(分辨率的分母)

    • 填料基架颗粒直径的选择(颗粒直径越小峰越尖)

      • 良好的柱填装技术(层析柱的柱效)

      • 目标分子的体积大小,溶液的粘度

      • 运行的流速

      • 图片