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分辨率/分离系数RsResolution
- 表彰两个峰的分离度出峰差和峰宽进行比较Rs=1表明2个峰刚好分离可节约时间Rs1分离度不行
- ![[层析分辨率.jpg]]
- 分辨率优化的两个方向
- 选择性(分辨率分子)
- 填料配基的选择
- 目标分子和吸附配基的结合条件优化(结合液性质)
- 分离条件优化(洗脱液性质和洗脱梯度)
- ![](media/8ac625320d42c79af6d9460116f76d40.jpeg)图片
![](media/fa5b97ed3ddfa1f698b35aefb1939bf9.jpeg)
- 有效性(分辨率的分母)
- 填料基架颗粒直径的选择(颗粒直径越小峰越尖)
- 良好的柱填装技术(层析柱的柱效)
- 目标分子的体积大小,溶液的粘度
- 运行的流速
- ![](media/2f8c600128e09249491e557e8b90f78c.jpeg)图片