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载量(吸附层析)

-   单位体积填料吸附的样品量

-   影响因素

    -   样品性质:分子量、形状、带电性、疏水性、标签暴露

    -   填料性质:配基类型和配基密度

    -   操作条件pH、盐浓度、添加剂、样品浓度、温度、流速

-   ![](media/15b7e65fc950f6115f40a2caa3041053.jpeg)图片

  • 载量的测定(可同时进行缓冲液优化)

    • 静态载量测定

      • 一般研发阶段进行测定

        • 可进行缓冲液优化

        • 图片

  • 动态载量测定

    • 一般生产规模进行测定

      • 根据动态载量来选择柱床体积

        • 图片