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载量(吸附层析)
- 单位体积填料吸附的样品量
- 影响因素
- 样品性质:分子量、形状、带电性、疏水性、标签暴露
- 填料性质:配基类型和配基密度
- 操作条件pH、盐浓度、添加剂、样品浓度、温度、流速
- ![](media/15b7e65fc950f6115f40a2caa3041053.jpeg)图片
>
- 载量的测定(可同时进行缓冲液优化)
- 静态载量测定
- 一般研发阶段进行测定
- 可进行缓冲液优化
- ![](media/4cd76f7545b26ede575a1632ad34fdb1.jpeg)图片
>
- 动态载量测定
- 一般生产规模进行测定
- 根据动态载量来选择柱床体积
- ![](media/85559fa568c19430fbd07e7b4c26554b.jpeg)图片
> ◊