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- Vo：外水体积/空流体积，填料分子外的体积，根据几何学原理，体积大约占柱体积的1/3，精确测量使用2000KD的葡聚糖 - Vi：内孔体积/内水体积，填料分子内的体积， - Vt：总液体体积 - Vs：固相凝胶体积，大约占柱体积的5% - Vc:柱体积，根据柱高和直径计算得到，又称柱床体积 - Ve：保留体积/洗脱体积，又叫VR - 体积压缩比：
