You can not select more than 25 topics Topics must start with a letter or number, can include dashes ('-') and can be up to 35 characters long.

471 B

This file contains ambiguous Unicode characters!

This file contains ambiguous Unicode characters that may be confused with others in your current locale. If your use case is intentional and legitimate, you can safely ignore this warning. Use the Escape button to highlight these characters.

构建细胞/工程菌时需要考虑纯化

表达位置

分胞内、胞外

hGH为分泌间质表达

表达位置决定了破碎的方法

冻融目的

使细胞松散

超滤

先超滤后盐析,因为体积大

经验值目的分子大分子÷膜孔5KDhGh=1.5倍

盐析

破坏蛋白亲水性水化膜,暴露疏水核

影响因素

蛋白浓度,体系中盐的浓度

过饱和盐:全部蛋白

不饱和盐:有目的筛选