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分胞内、胞外
hGH为分泌间质表达
表达位置决定了破碎的方法
使细胞松散
先超滤后盐析,因为体积大
经验值:目的分子(大分子)÷膜孔(5KD,hGh)=1.5倍
破坏蛋白亲水性水化膜,暴露疏水核
影响因素
蛋白浓度,体系中盐的浓度
过饱和盐:全部蛋白
不饱和盐:有目的筛选