You can not select more than 25 topics Topics must start with a letter or number, can include dashes ('-') and can be up to 35 characters long.

86 lines
1.1 KiB

This file contains ambiguous Unicode characters!

This file contains ambiguous Unicode characters that may be confused with others in your current locale. If your use case is intentional and legitimate, you can safely ignore this warning. Use the Escape button to highlight these characters.

0.04M NaCl结合能力减弱杂质流穿
0.1M NaCl目的蛋白
M NaCl洗脱结合杂蛋白
> DEAE1
> 2021年6月24日 21:48
- DEAE1
- 目的
> 捕获目的蛋白
- 层析原理
- 离子交换(弱阴离子交换),低盐上样,高盐洗脱
- 层析柱型号
柱内直径cm
柱高cm
柱床体积L
柱床高度cm
上样量mg/ml
蛋白处理范围g
INDEX 200/500
20
50
8.5±0.5
25±5
25\~50
200\~450
>
- 填料信息
- 名称DEAE Sephrose F.F
- 更换时间
- 柱效要求
2014年
- 每米理论塔板数≥2000
□ 对称性0.8\~1.2
- 缓冲液
- 平衡缓冲液
- 0.3mol/L Tris-HCl-30m mol/L EDTA
- 0.04mol/L NaCl-10m mol/L Tris-HCl-1m mol/L EDTA
- 样品调节缓冲液
- 0.04mol/L NaCl -10m mol/L Tris-HCl-1m mol/L EDTA
- 冲洗缓冲液
- 0.04mol/L NaCl-10m mol/L Tris-HCl-1m mol/L EDTA
- 洗脱缓冲液
- 0.1mol/L NaCl-10m mol/L Tris-HCl-1m mol/L EDTA
- Q&A
- 装柱比较困难
> 分区 层析 的第 1 页