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DEAE2

2021年6月24日 21:49

  • DEAE2

  • 目的

捕获目的蛋白

  • 层析原理

    • 离子交换(弱阴离子交换)

    • 层析柱型号

      柱内直径cm

      柱床体积L

      柱床高度cm

      上样量mg/ml

      蛋白处理范围g

      INDEX 100/500

      10

      3.0±0.5

      40±5

      15~40

      37.5~140

      INDEX 140/500

      14

      5.5±0.5

      35±5

      15~40

      75~200

  • 填料信息

    • 名称DEAE Sephrose F.F

      • 更换日期

  • 柱效要求

    2014年

  • 每米理论塔板数≥2000

    □ 对称性0.8~1.2

  • 缓冲液

    • 平衡缓冲液

      • 200m mol/L PB

        • 5m mol/L PB
      • 样品调节缓冲液

        • 5m mol/L PB
      • 冲洗缓冲液

        • 5m mol/L PB
      • 洗脱缓冲液

        • 0.02mol/L NaCl-5m mol/L PB

        • 0.05mol/L NaCl-5m mol/L PB

        • 0.08mol/L NaCl-5m mol/L PB

分区 层析 的第 1 页